Real-time Polymerase Chain Reaction:Article Title: A post-injury immune challenge with lipopolysaccharide following adult traumatic brain injury alters neuroinflammation and the gut microbiome acutely, but has little effect on chronic outcomes.
Article Snippet: RNA purity was determined using a QIAxpert spectrophotometer then converted to cDNA via a QuantiTect® Reverse Transcription Kit (Qiagen). .. High-throughput qPCR analysis was carried out by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility, using the Fluidigm BioMark HDTM system (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A 24-gene panel were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il1β (Mm00434228_m1), Il1α (Mm00439620_m1), Tnfα (Mm99999068_m1), Il6 (Mm00446190_m1), Il10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
Article Title: A post-injury immune challenge with lipopolysaccharide following adult traumatic brain injury alters neuroinflammation and the gut microbiome acutely, but has little effect on chronic outcomes
Article Snippet: RNA purity was determined using a QIAxpert spectrophotometer then converted to cDNA via a QuantiTect® Reverse Transcription Kit (Qiagen). .. High-throughput qPCR analysis was carried out by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility, using the Fluidigm BioMark HDTM system (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A 24-gene panel were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il1β (Mm00434228_m1), Il1α (Mm00439620_m1), Tnfα (Mm99999068_m1), Il6 (Mm00446190_m1), Il10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
Article Title: Modelling lung infection with Klebsiella pneumoniae after murine traumatic brain injury.
Article Snippet: RNA purity was measuring using the QIAxpert spectrophotometer, then converted to cDNA using the QuantiTect® Reverse Transcription Kit (Qiagen). .. High throughput qPCR was performed by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility using the Fluidigm BioMark HDTM (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A panel of 24 genes were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il-1β (Mm00434228_m1), Il-1α (Mm00439620_m1), Tnf-α (Mm99999068_m1), Il6 (Mm00446190_m1), Il-10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_ m1).
Article Title: Modelling lung infection with Klebsiella pneumoniae after murine traumatic brain injury
Article Snippet: RNA purity was measuring using the QIAxpert spectrophotometer, then converted to cDNA using the QuantiTect® Reverse Transcription Kit (Qiagen). .. High throughput qPCR was performed by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility using the Fluidigm BioMark HDTM (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A panel of 24 genes were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il-1β (Mm00434228_m1), Il-1α (Mm00439620_m1), Tnf-α (Mm99999068_m1), Il-6 (Mm00446190_m1), Il-10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
Gene Expression:Article Title: A post-injury immune challenge with lipopolysaccharide following adult traumatic brain injury alters neuroinflammation and the gut microbiome acutely, but has little effect on chronic outcomes.
Article Snippet: RNA purity was determined using a QIAxpert spectrophotometer then converted to cDNA via a QuantiTect® Reverse Transcription Kit (Qiagen). .. High-throughput qPCR analysis was carried out by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility, using the Fluidigm BioMark HDTM system (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A 24-gene panel were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il1β (Mm00434228_m1), Il1α (Mm00439620_m1), Tnfα (Mm99999068_m1), Il6 (Mm00446190_m1), Il10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
Article Title: A post-injury immune challenge with lipopolysaccharide following adult traumatic brain injury alters neuroinflammation and the gut microbiome acutely, but has little effect on chronic outcomes
Article Snippet: RNA purity was determined using a QIAxpert spectrophotometer then converted to cDNA via a QuantiTect® Reverse Transcription Kit (Qiagen). .. High-throughput qPCR analysis was carried out by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility, using the Fluidigm BioMark HDTM system (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A 24-gene panel were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il1β (Mm00434228_m1), Il1α (Mm00439620_m1), Tnfα (Mm99999068_m1), Il6 (Mm00446190_m1), Il10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
Article Title: Modelling lung infection with Klebsiella pneumoniae after murine traumatic brain injury.
Article Snippet: RNA purity was measuring using the QIAxpert spectrophotometer, then converted to cDNA using the QuantiTect® Reverse Transcription Kit (Qiagen). .. High throughput qPCR was performed by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility using the Fluidigm BioMark HDTM (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A panel of 24 genes were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il-1β (Mm00434228_m1), Il-1α (Mm00439620_m1), Tnf-α (Mm99999068_m1), Il6 (Mm00446190_m1), Il-10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_ m1).
Article Title: Modelling lung infection with Klebsiella pneumoniae after murine traumatic brain injury
Article Snippet: RNA purity was measuring using the QIAxpert spectrophotometer, then converted to cDNA using the QuantiTect® Reverse Transcription Kit (Qiagen). .. High throughput qPCR was performed by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility using the Fluidigm BioMark HDTM (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A panel of 24 genes were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il-1β (Mm00434228_m1), Il-1α (Mm00439620_m1), Tnf-α (Mm99999068_m1), Il-6 (Mm00446190_m1), Il-10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
High Throughput Screening Assay:Article Title: Modelling lung infection with Klebsiella pneumoniae after murine traumatic brain injury.
Article Snippet: RNA purity was measuring using the QIAxpert spectrophotometer, then converted to cDNA using the QuantiTect® Reverse Transcription Kit (Qiagen). .. High throughput qPCR was performed by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility using the Fluidigm BioMark HDTM (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A panel of 24 genes were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il-1β (Mm00434228_m1), Il-1α (Mm00439620_m1), Tnf-α (Mm99999068_m1), Il6 (Mm00446190_m1), Il-10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_ m1).
Article Title: Modelling lung infection with Klebsiella pneumoniae after murine traumatic brain injury
Article Snippet: RNA purity was measuring using the QIAxpert spectrophotometer, then converted to cDNA using the QuantiTect® Reverse Transcription Kit (Qiagen). .. High throughput qPCR was performed by the Monash Health Translation Precinct (MHTP) Medical Genomics Facility using the Fluidigm BioMark HDTM (Standard BioTools Inc., San Francisco, CA, USA) in a Fluidigm 192.24 Dynamic ArrayTM Integrated Fluidic Circuit for Gene Expression. .. A panel of 24 genes were analyzed, including the housekeeping genes (HKGs) Ywhaz (Mm01722325_m1), Ppia (Mm02342430_g1) and Hprt (Mm03024075_m1) (Zamani et al., 2020), as well as the following cytokines, chemokines, immune cell markers, and immune-regulatory genes: Il-1β (Mm00434228_m1), Il-1α (Mm00439620_m1), Tnf-α (Mm99999068_m1), Il-6 (Mm00446190_m1), Il-10 (Mm01288386_m1), Ccl2 (Mm00441242_m1), Ccr2 (Mm00438270_m1), Cxcl2 (Mm00436450_m1) and Cxcr2 (Mm99999117_s1), Cd45 (Mm01293577_m1) and Cd86 (Mm00444543_m1), Gfap (Mm01253033_m1), Aldh1l1 (Mm03048957_m1), Arg1 (Mm00475988_m1), Tmem119 (Mm00525305_m1), Trem2 (Mm04209424_g1), Nos2 (Mm00440485_m1), Tgf1 (Mm01227699_m1), Mapk1 (Mm00442479_m1), Nfkb1 (Mm00476361_m1), and Hmox1 (Mm00516005_m1).
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